اثر تعلیق مکانیکی اندام تحتانی بر بیان ژن‌های TRAF6 و MuRF1 در عضلۀ نعلی موش‌های صحرایی نر ویستار

نوع مقاله : علمی - پژوهشی

نویسندگان

1 گروه علوم ورزشی، دانشکدۀ ادبیات و علوم انسانی، دانشگاه ولیعصر رفسنجان، رفسنجان، ایران

2 گروه فیزیولوژی ورزشی، دانشکدۀ ادبیات و علوم انسانی، دانشگاه آزاد اسلامی، کرمان، ایران

3 گروه علوم ورزشی، دانشکدۀ ادبیات و علوم انسانی، دانشگاه تربیت مدرس، تهران، ایران

چکیده

هدف: به‌دلیل ابهامات موجود در زمینۀ فرایندهای رشد و حفظ عضلات اسکلتی به‌ویژه آتروفی ناشی از بی‌تحرکی، مطالعۀ حاضر، به‌منظور بررسی تأثیرات تعلیق مکانیکی اندام تحتانی بر بیان ژن‌های آتروژنیکی در عضلۀ نعلی موش‌های صحرایی انجام گرفت.
روش‌ها: بدین‌منظور 10 سر موش صحرایی نژاد ویستار در دو گروه تعلیق (5=n) و کنترل (5=n) قرار گرفتند و موش‌های صحرایی گروه تعلیق به مدت دو هفته در همین شرایط نگه‌داشته شدند. پس از 14 روز عضلۀ نعلی آن‌ها استخراج گردید و بیان ژن‌های TRAf6 و MuRF1 به روش real-time-Pcr اندازه‌گیری شد.
 نتایج: نتایج نشان داد که پس از 14 روز تعلیق اندام تحتانی وزن نسبی عضلۀ نعلی به‌طور معنا‌داری کاهش یافته بود (009/0=P). به‌علاوه، بیان ژن TRAF6 (033/0=P) به‌طور معنا‌داری افزایش یافته بود، ولی افزایش MuRF1 به لحاظ آماری معنا‌دار نبود (061/0=P).
 نتیجه‌گیری: با توجه به نتایج پژوهش حاضر می‌توان گفت که TRAF6 و عامل پایین‌دست آن، MuRF1 می‌توانند در تنظیم تودۀ عضلانی در شرایط کاهش فعالیت عصبی عضلانی و تعلیق اندام‌ها درگیر باشند و به‌عنوان عامل منتخب مقابله با کاهش تودۀ عضلانی در وضعیت‌هایی مانند بستری شدن ورزشکاران پس از آسیب‌های ورزشی یا کاهش بار اندام پس از آسیب‌دیدگی درنظر گرفته شوند.

کلیدواژه‌ها


عنوان مقاله [English]

The Effect of mechanical unloading on TRAF6 and MuRF1 genes expression in soleus muscle of male Wistar rats

نویسندگان [English]

  • abdolreza kazremi 1
  • zahra masoumpor 2
  • amirbahador dakhili 3
  • Alireza Zangiabadi 2
  • Iman fathi 1
1 Sport sciences, Faculty of Humanity and Litrutur, Vali-E-Asr University of Rafsanjan, Rafsanjan, Iran
2 Exercise physiology, Faculty of Humanity and Litrutur, Azad University of Kerman, Kerman, Iran
3 Sport sciences, Faculty of Humanity and Litrutur, Tarbiat Modares University, Tehran, Iran
چکیده [English]

Purpose: Because of exiting uncertainties in growth and maintenance of muscle mass processes, particular atrophy inducing inactivity, the aim of the present study was to investigate changes in the expression of atrogenic genes in the muscle of the rat in which the hind-limbs were kept under unloaded conditions.
Methods: For this purpose, 10 male Wistar rats were assign in two groups of suspension (n=5) and control (n=5) and the suspension group rats were kept for two weeks in this conditions. After 14 days, the soleus muscle was extracted and the expression of TRAf6 and MuRF1 genes was measured by real-time-Pcr method.
Results: The results of this study showed that after 14 days of hind-limb suspension, the relative weight of soleus muscle was significantly decreased (P=0.009). In addition, the expression of TRAF6 gene was significantly increased (P=0.033), but MuRF1 was not statistically significant (P=0.061).
Conclusion: Therefore, according to the results of this study, it can be stated that TRAF6 and its downstream factor, MuRF1, can be involved in the regulation of muscle mass in conditions of reduced muscular activity and mechanical unloading and as candidates for controlling muscle mass in conditions such as athlete's hospitalized after sports injuries or hind-limb unloading after injury to be considered.

کلیدواژه‌ها [English]

  • Keywords: Hind-limb suspension
  • skeletal muscle atrophy
  • Atrogenic genes
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  • تاریخ دریافت: 19 شهریور 1397
  • تاریخ بازنگری: 19 مرداد 1398
  • تاریخ پذیرش: 01 مهر 1398
  • تاریخ اولین انتشار: 01 اسفند 1399
  • تاریخ انتشار: 01 اسفند 1399