تاثیر شش هفته تمرین تناوبی شدید بر مقادیر بیان ژن MMP-9، eNOS و نسبت VEGF به COL-18 در رت‌های مبتلا به آنفارکتوس میوکارد

نوع مقاله : علمی - پژوهشی

نویسنده

دانشگاه تهران

چکیده

هدف تحقیقانفارکتوس میوکارد، مرگ سلولی بخشی از عضله قلب به علت از بین رفتن جریان خون است. فرایند مولکولی افزایش تراکم مویرگی در پاسخ به فعالیت هنوز معلوم نیست. لذا این پژوهش با هدف ارزیابی اثر شش هفته شدت تمرین تناوبی شدید بر مقادیر MMP-9 و eNOS و نسبت VEGF به COL-18 در رت های مبتلا به آنفارکتوس میوکارد انجام گرفت.روش تحقیق18سررت نر نژاد ویستار 10هفته ای با وزن 250 تا 300گرم در دو گروه تمرین تناوبی شدید(60 دقیقه دویدن تناوبی روی تردمیل و هر تناوب شامل چهار دقیقه با شدت 85-90 و دو دقیقه بازیافت فعال با شدت 50-60 درصد VO2max، چهار روز در هفته و به مدت شش هفته) و گروه کنترل(بدون مداخله تمرین) قرار گرفتند. بعد از آن رت ها به انفارکتوس میوکارد مبتلا و بیان ژن های MMP-9 و eNOS وVEGF و COL-18 توسط PCR Real time بررسی شد. داده ها با استفاده از نرم افزار نسخه spss18(α≥0.05) به روش آماری T مستقل تجزیه و تحلیل شدند. نتایجنتایج آزمون تی مستقل نشان داد مقادیر MMP-9 گروه تمرین تناوبی شدید (541/3) به طور معناداری بیشتر از گروه کنترل(002/1)(0.012=P) و مقادیر eNOS گروه تمرین تناوبی شدید(23/4) نیز به طور معناداری بیشتر از گروه کنترل (68/3) است(014.0=P) اما افزایش نسب VEGF به COL-18 گروه تمرین تناوبی شدید(mg/ml856/1) نسبت به کنترل(mg/ml245/1) معنادار نیست(0.263=P) .بحث و نتیجه گیری به طور کلی شش هفته تمرین تناوبی شدید باعث افزایش عوامل آنژیوژنز و بهبود عملکرد قلبی در رت های نر نژاد ویستار پس از وقوع آنفارکتوس میوکارد می‌شود.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of 6 weeks high intensity interval training on selected factors of caadiac angiogenesis in rats with myocardial infarction.

چکیده [English]

Aim
myocardial infarction(MI) is destruction and cell Death in part of the myocardial that it Happens During an Ischemia. Evidence suggests that exercise training Increased capillary density in Skeletal and myocardial muscles presumably. But Cell process In response to activity and appropriate Intensity is not clear. Vascular endothelial growth factor(VEGF) and endostatin and also their common receptor(FLT-1) are the most important factors involved in angiogenesis.
Methodology
in this study we investigated the effect of 6 weeks of high intensity interval training on changes of these factors in 12 male Wistar rats with 10-week old with myocardial infarction in two groups, experimental(60 minutes interval running on treadmill, 4 minutes running with 85-90% of VO2max , and 2 minutes recovery with 50-60% VO2max and four days a week per six weeks) and control(no exercise).
Result
The results show a positive and significant increase in FLT-1 and VEGF and no significant changes in collagen 18 gene expression during HIIT training.
Conclusion
Overall its results conforms that HIIT training can effective on angiogenesis factors after MI.

کلیدواژه‌ها [English]

  • angiogenesis
  • myocardial infarction- high intensity interval training
Nordlie MA, Wold LE, Kloner RA. Genetic contributors toward increased risk for ischemic heart disease. J Mol Cell Cardiol. 2005:39(4):667-79.
Fukuda Sh, Kaga Sh, Sasaki H, Zhan L, Zhu L, Otani H , et al. Angiogenic signal triggered by ischemic stress induces myocardial repair in rat during chronic infarction. J Mol Cell Cardio. 2004:36(4):547-559.
Nourshahi M, Taheri chadorneshin H, Ranjbar K. [The stimulus of angiogenesis during exercise and physical Activity (Persian)]. Quarterly of the Horizon of Medical Sciences. 2013:18(5):286-296.
Gavin TP, Stallings HW, Zwetsloot KA, Westerkamp LM, Ryan NA, Moore RA, et al. Lower capillary density but no difference in VEGF expression in obese vs lean young skeletal muscle in humans. J Appl Physiol(1985). 2005:98(1):315-21.
Mooren F, Völker K. Molecular and Cellular Exercise Physiology. Human Kinetics. 2004: 451-7.
Heldin CH, Westermark B. Mechanism of action and in vivo role of platelet-derived growth factor. J Physiol Rev. 2004:79(4):1283-316.
Van Hinsbergh VWM, Koolwijk P. Endothelial sprouting and angiogenesis matrix metalloproteinases in the lead. J Cardiovascular Research.2008:78:203-12.
Gu JW, Gadonski G, Wang J, Makey I, Adair TH. Exercise increases endostatin in circulation of healthy volunteers. J BMC Physiol. 2004:16(4):2.
Holloway TM, Bloemberg D, da Silva ML, Simpson JA, Quadrilatero J, Spriet LL. High Intensity Interval and Endurance Training Have Opposing Effects on Markers of Heart Failure and Cardiac Remodeling in Hypertensive Rats. PLoS One. 2015:10(3):e0121138.
Laursen PB, Jenkins DG. The scientific basis for high- intensity interval training opti mizing training programmes and maximizing performance in highly trained endurance athletes. J Sports Med. 2002:32(1):53-73.
Truijens MJ, Toussaint HM, Dow J, Levine BD. Effect of high-intensity hypoxic training on sea-level swimming performances. J Appl Physiol (1985). 2002:94(2):733-43.
Padilla J, Harris RA, Rink LD, Wallace PJ. Characterization of the brachial artery shear stress following walking exercise. J Vasc Med. 2008:13(2):105-11.
Burgomaster KA, Howarth KR, Phillips SM, Rakobowchuk M, MacDonald MJ, McGee SL, et al. Similar metabolic adaptations during exercise after low volume sprint interval and traditional endurance training in humans. J Physiol. 2008:586(1):151-160.
Rodas G, Ventura JL, Cadefau JA, Cusso R, Parra J. A short training programme for the rapid improvement of both aerobic and anaerobic metabolism. Eur J Appl Physiol. 2000:82(5-6):480-6.
Edge J, Bishop D, Hill-Haas S, Dawson B, Goodman C. Comparison of muscle buffer capacity and repeatedsprint ability of untrained, endurance-trained and team-sport athletes. Eur J Appl Physiol. 2006:96(3):225-34.
Weston KS, Wisløff U, Coombes J. High-intensity interval training in patients with lifestyle-induced cardiometabolic disease: a systematic review and meta-analysis. Br J Sports Med. 2014:48(16):1227-34.
Hamzeh zadeh brojeni A, Nazar Ali P, Naghibi S. [The Effect of High Intensity Interval Training(HIIT) on aerobic and anaerobic some indicators of iranian women's national teams of basketball players (Persian)]. Exercise Physiology. 2012:5(4):35-48.
Martin J, Gibala M G, McGee SL. Metabolic Adaptations to Short-term High-Intensity Interval Training: A Little Pain for a Lot of Gain? Sport Sci. 2008:36(2):58-63.
Donovan G O, Owen A, Bird SR, Kearney EM, Nevill AM, Jones DW, et al. Changes in cardiorespiratory fitness and coronary heart disease risk factors following 24 wk of moderate- or high-intensity exercise of equal energy cost. J Appl Physiol. 2005:98(5):1619-25.
Nazari M, Kordi MR, Choobineh S. [The Effect of High Intensity Interval Training (HIIT) on Gelatinase-A (MMP-2) Serum Levels and Muscle Damage Indices in Young Sedentary (Persion)]. J Arak Medical University. 2015:18(94):78-86.
Danzig V, Mikova B, Kuchynka P, Benakova H, Zima T, Kittnar O, et al. Levels of circulating biomarkers at rest and after exercise in coronary artery disease patients. Physiol Res. 2010:59(3):385-92.
Kraljevic J, Marinovic J, Pravdic D, Zubin P, Dujic Z, Wisloff U, et al. Aerobic interval training attenuates remodelling and mitochondrial dysfunction in the post-infarction failing rat heart. Cardiovasc Res. 2013;99(1):55-64.
Morten A, Hoydal MA, Wisloff U, Kemi OJ, Ellingsen O. Running speed and maximal oxygen uptake in rats and mice: practical implications for exercise training. Eur J Cardiovasc Prev Rehabil. 2007:14(6):753-60.
Wisloff U, Helgerud J, Kemi OJ, Ellingsen O. Intensity-controlled treadmill running in rats: VO2 max and cardiac hypertrophy. Am J Physiol Heart Circ Physiol. 2000:280(3):1301-10.
Kemi OJ, Haram PM, Loennechen JP, Osnes JB, Skomedal TU, Wisløff U, Ellingsen O. Moderate vs high exercise intensity: Differential effects on aerobic fitness, cardiomyocyte contractility, and endothelial function. Cardiovasc Res. 2005:67(1):161-72.
Hudlicka O, Brown MD. Adaptation of Skeletal Muscle Microvasculature to Increased or Decreased Blood Flow Role of Shear Stress Nitric Oxide and Vascular Endothelial Growth Factor. J Vasc Res. 2009:46(5):504-12.
Prior BM, Yang HT, Terjung RL. What makes vessels grow with exercise training? J Appl Physiol(1985). 2004:97(3):1119-28.
Koos BJ. Adenosine A2a receptors and O2 sensing in development. Am J Physiol Regul Integr Comp Physiol. 2011:301(3):601-22.
Ribatti D, Crivellato E. Mast cells, angiogenesis, and tumour growth. Biochim Biophys Acta. 2012:1822(1):2-8.
Folkman J. Fundamental concepts of the angiogenic process. Curr Mol Med. 2003:3(7): 643-51.
Visartaite A, Vainoras A, Sedekerskis V, Poderys J. The influence of aerobics exercise to cardiovascular functional parameters of 30-40 year old women. Medicina (Kaunas). 2004:40(5):451-8.
Rawlins J, Bhan A, Sharma S. Left ventricular hypertrophy in athletes. Eur J Echocardiogr. 2009:10(3):350-6.