تاثیر تمرین تناوبی شدید و تمرین تداومی بر عملکرد سلولهای بنیادی و ظرفیت خود نوزایی سلول های میوکاردموش های صحرایی

نوع مقاله : علمی - پژوهشی

نویسندگان

1 دانشکده تربیت بدنی و علوم ورزشی، دانشگاه تهران، تهران، ایران

2 موسسه تحقیقاتی هیستوژنوتک پاسارگاد، تهران، ایران

چکیده

هدف: نشان داده شده که فعالیت ورزشی در بیشتر گونه های جانوری به افزایش و بهبود شاخص­های عملکردی قلبی منجر می شود. به نظر می­رسد که به دنبال انواع فعالیت ورزشی ظرفیت خود نوزایی قلبی از طریق افزایش تشکیل کاردیومیوسیت­های جدید افزایش می­یابد. مطالعه حاضر تاثیر تمرینات تناوبی شدید و تداومی بر عملکرد سلول های بنیادی و ظرفیت خود نوزایی قلبی را بررسی می­کند.روش شناسی: 24 عدد رت ویستار نر بالغ به سه گروه تمرین تناوبی شدید ،تداومی و کنترل تقسیم شدند. پروتکل های تمرینی 5 روز در هفته و 6 هفته اجرا شد. بافت قلب استخراج و مقادیر c-Kit و  Ki67 به روش ایمونوهیستوشیمی و بیان ژن  Nkx2.5به روش RealTime-PCR آنالیز شد. روش اماری تحلیل واریانس یک طرفه با معناداری  )05/0(P≤ استفاده شد. نتایج: افزایش معنادار سلول­های مثبت c-Kit در گروه تمرین HIIT )00/0(P≤ و در گروه تداومی )0۱۸/0(P≤  مشاهده شد که این افزایش در گروه HIIT بیشتر بود. افزایش معنادار سلول های مثبتKi67  در گروه تمرین HIIT )00/0(P≤ و گروه تداومی )05/0(P≤ مشاهده شد که این افزایش در گروهHIIT  بیشتر بود. همچنین افزایش معنادار بیان ژن  Nkx2.5در گروه تمرین HIIT )015/0(P≤و در گروه تداومی )03/0(P≤ مشاهده شد که این افزایش در گروه تداومی بیشتر بود. بحث و نتیجه گیری:به نظر می رسدتمرینات ورزشی از طریق افزایش تکثیر سلول­های بنیادی در قلب بازسازی قلب را آغاز کرده و متعاقب آن تمایزc-Kit را فعال و بیان ژن Nkx2.55 را که دو عامل اصلی رونویسی اولیه دودمان قلب هستند افزایش می­دهد؛ که موجب تولید سلولهای جدید قلب می­شود. 

کلیدواژه‌ها


عنوان مقاله [English]

The effect of high intensity interval and continues training on cardiac stem cells function and myocardial regeneration capacity in male rats

نویسندگان [English]

  • AREZOO ESKANDARI SHAHRABI 1
  • rahman sori 1
  • siroos chobineh 1
  • zohre mazaheri tirani 2
1 Faculty of Physical Education and sport science, Tehran University, Tehran, Iran
2 Histogenotac institute, Tehran, Iran
چکیده [English]

Purpose: Adult cardiac stem cells have ability to regenerate and repair cardiac cells through proper stimulation. Cardiac regeneration capacity decreases with ageing, while physical activities play a positive role in this case. The aim of this study was the effect of high intensity interval and continues training on cardiac stem cells function and myocardial regeneration capacity in male rats.
Methods: Twenty-four Vistar male rats (365.6±13.3 g, 6-8 month) were divided into three groups HIIT (high intensity) continuous and control. The training protocol was performed 5 sessions per week for 6 weeks. The heart rats were extracted and c-Kit and Ki67 values were analyzed by Immunohistochemistry and Nkx2.5 gene expression for cardiac stem cells was measured by Real Time-PCR. The data were analyzed by one-way ANOVA (P≤0.05).
Results: According to the results of this study, there was a significant increase in c-Kit cells in the HIIT (P=0.001) and in the continuous group (P=0.018) and Significant increase in positive Ki67 cells in the HIIT group (P=0.001) and continuous group (P=0.05). This increase was greater in the HIIT group. As well as a significant increase was observed in the Nkx2.5 gene expression in the HIIT training group (P=0.015) and in the continuous group (P=0.03) which increased in the continuous group was grater.
Conclusion: The results showed that the intensity of training is more effective for cardiac regeneration adaptation and HIIT have more positive effects on differentiation of cardiac stem cells.

کلیدواژه‌ها [English]

  • HIIT Training
  • continuous Training
  • Cardiac Stem Cells
  • c
  • Kit
  • Nkx2.5
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  • تاریخ دریافت: 22 دی 1396
  • تاریخ بازنگری: 19 بهمن 1399
  • تاریخ پذیرش: 11 دی 1399
  • تاریخ اولین انتشار: 11 دی 1399
  • تاریخ انتشار: 01 تیر 1398